ISSN 0976-4550  
DOI: 10.21276/Ijabpt  
 
 
 
Peer Reviewed Online International Journal of Applied Biology and Pharmaceutical Technology www.ijabpt.com
 

ANALYTICAL STANDARDS FOR THE ROOT TUBERS OF ACONITUM HETEROPHYLLUM WALL. EX ROYLE. -A RARE MEDICINAL PLANT OF SIKKIM HIMALAYAN REGION  Page No 43 to 51, Volume-8, Issue-3, July-Sept-2017

Shashi Verma and L.K.Nath

Standardization of herbal drugs is the need of the hour as the use and practice of traditional herbal drugs has increased tremendously. The main objective of the present study is to standardize the root tubers of Aconitum heterophyllum as per pharmacopoeial testing protocol which includes powder microscopy, physico-chemical screening, TLC profile and chromatographic studies. Preliminary phytochemical investigations revealed the presence of primary and secondary metabolites as carbohydrates, phenolics in methanolic and aqueous extract. The alkaloids may be present in chloroform extract .Result also indicated that, petroleum ether and chloroform extracts of Aconitum heterophyllum showed presence of steroids and triterpenoids. TLC profiling of all plant extracts also give an idea about the presence of these phytochemicals. Rf (Retention factor) value of different phytochemicals provide valuable clue regarding their polarity and selection of solvents for separation of phytochemicals. Concentrating on results obtained in TLC, the column chromatographic studies were set.  The fraction containing search here was flavonoids as, quercetin. Performing TLC of each individual fraction, same Rf value fractions were collected& concentrated during column chromatography.  

Key Words: Aconitum heterophyllum, phytochemical screening, TLC Profiling, & Retention factor (Rf.), Column chromatography

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