ISSN 0976-4550  
DOI: 10.21276/Ijabpt  
 
 
 
Peer Reviewed Online International Journal of Applied Biology and Pharmaceutical Technology www.ijabpt.com
 

COMPARISON OF POLYPHENOLS, FLAVANOIDS, ANTIOXIDANT AND FREE RADICAL SCAVENGING CONTENT OF FREEZE DRIED WHEATGRASS EXTRACT FROM THREE DIFFERENT WHEAT SPECIES.  Page No 98 to 106, Volume-8, Issue-1, Jan-Mar-2017   DOI: 10.21276/ijabpt.2017.8.1.14

Amandeep Saini, Shweta Sinha and Jagtar Singh

Oxidative stress is the leading cause of numerous age-related and chronic disorders that can be controlled by using antioxidants. Thus, there is growing concern to screen natural antioxidants from plant source that will enhance the nutritional quality of human foods to prevent disease and mitigate age-related symptoms. Wheatgrass (WG) has a potent antioxidant efficacy therefore, the present study is aimed to evaluate and compare the phytoconstituents from T. dicoccum, T. durum and T. aestivum. The WG were freeze dried and methanolic extract were prepared. To test antioxidant activity, total phenolic, flavanoid and antioxidant content along with free radical scavenging assay were performed in order to provide natural antioxidants in food and pharmaceutical supplements. The extracts were prepared from day 6, 9, 12, 15 WG from all three wheat species. Our results found that T. dicoccum have highest polyphenol, flavanoid and antioxidant contents with 192 GAE/g, 109 QE/g and 116 AAE/g dry weight of the dry extract in day 15 sample. The IC50 value in DPPH assay was 6.32 µg/mL for T. dicoccum followed by 9.2 µg/mL for T. durum and 11 µg/mL for T. aestivum respectively. In conclusion, comparing three wheat species of WG our result showed maximum antioxidant and free radical scavenging activity in T. dicoccum followed by T. durum and T. aestivum in day 15 sample that can be explored for therapeutic applications.

Key words: Wheatgrass, total antioxidant, total flavanoid, total phenolics, 2,2-diphenyl-1-picrylhydrazyl

Full Text Pdf
 
 
 
Copyright @ 2010 www.ijabpt.com All rights reserved
Web Counter