ISSN 0976-4550  
DOI: 10.21276/Ijabpt  
 
 
 
Peer Reviewed Online International Journal of Applied Biology and Pharmaceutical Technology www.ijabpt.com
 

ISOLATION, BIOCHEMICAL CHARACTERIZATION AND METABOLIC FINGERPRINTING OF RHIZOBIUM FROM ROOT NODULES OF CLITORIATERNATEA. Page No 19 to 30, Volume-8, Issue-1, Jan-Mar-2017   DOI: 10.21276/ijabpt.2017.8.1.3

Dr.Mrs. Sonali Nigam and Priya Dipti Xaxlo

ABSTRACT: One of the unique ability of leguminous plants is to establish symbiosis interaction with nitrogen fixing bacteria. The symbiotic association of such leguminous plants with rhizobia plays a major role in the agriculture crop production. The prime objective of this research paper was to isolate bacteria (Rhizobium) from root nodules of Clitoriaternatea. Therhizobial strains were further subjected to morphological, biochemical and metabolic characterization. Pure culture of rhizobial strains was isolated on selective YEMA medium. Antibiotic resistant pattern of all isolates was also evaluated against two antibiotics. In contrast, based on carbon utilization pattern a remarkable metabolic diversity was observed among therhizobial isolates recovered in the studies.

Key words: Root nodules, Rhizobium, Clitoria, Carbon source.

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