ISSN 0976-4550  
DOI: 10.21276/Ijabpt  
 
 
 
Peer Reviewed Online International Journal of Applied Biology and Pharmaceutical Technology www.ijabpt.com
 

EFFICIENT SHOOT INDUCTION AND PLANT REGENERATION OF PIGEON PEA [CAJANUS CAJAN (L.) MILLISP.] ICPL 87 VARIETY USING LEAF PETIOLE EXPLANTS. Page No 30 to 35, Volume-8, Issue-4, Oct-Dec-2017

Nirmala Nalluri and Vasavi Rama Karri

Pigeon pea or red gram is a high protein legume crop of semi-arid tropics and sub-tropics, which ranks sixth in production among the other legumes. It is used as a major source of protein (21%) in many countries like India. In the present study, an efficient in vitro regeneration protocol was developed for ICPL 87 variety of pigeon pea (Cajanus cajan L.) using leaf petiole explants. Leaf petiole explants of six day old seedlings were cultured on MS media supplemented with different concentrations of 6-Benzylaminopurine and α-napthaleneacetic acid for multiple shoot bud induction. MS media supplemented with 0.5 mg/l α-napthalene acetic acid + 2 mg/l 6-Benzylaminopurine hormonal concentrations induced highest number of shoot buds compared to other combinations. Induced multiple shoots were elongated on MS media fortified with 6-Benzylaminopurine, α-napthaleneacetic acid and Gibberellic acid hormonal combinations. Maximum number of shoots were elongated with hormonal combination of 1 mg/l 6-Benzylaminopurine + 0.2 mg/l α-napthaleneacetic acid + 3 mg/l Gibberellic acid. These well-elongated shoots were rooted on MS media enriched with different concentrations of Indole-3-butyric acid and maximum percentage of rooting was observed on MS media supplemented with 0.8 mg/l Indole-3-butyric acid concentration. Finally, these rooted plants were transferred to soil and vermiculate mixture in 1:1 ratio for hardening and acclimatization. The protocol standardized can be used for genetic transformation of pigeon pea to generate transgenic pigeon pea plants.

Key words: Pigeon pea, Organogenesis, Auxins, Cytokinins, Gibberellins

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