ISSN 0976-4550  
DOI: 10.21276/Ijabpt  
 
 
 
Peer Reviewed Online International Journal of Applied Biology and Pharmaceutical Technology www.ijabpt.com
 

EVALUATION OF HEPATOTOXICITY IN MICE BY 2,4- DICHLOROPHENOXY ACETIC ACID (2,4-D): A PROTECTIVE EFFECT OF MELATONIN  Page No 84 to 91, Volume-8, Issue-3, July-Sept-2017

Aparna Satapathy and Mandava V. Rao

As a selective herbicide, 2,4- dichlorophenoxy acetic (2,4-D) acid is worldwide used in  agricultural field. It has been associated with a variety of toxicities in rodents including man.The present study was performed to evaluate alterations in biochemical and antioxidant biomarkers of liver in adult male mice(Musmusculus) exposed to it at different concentrations (30, 60, 90 mg/kgbw) for 45 days daily and its mitigation by melatonin (10 mg/kgbw). Liver dysfunctions were confirmed by decrements in metabolic indices i.ephosphatases, adenosine triphosphatase, succinate dehydrogenase and total proteins. Similarly, various oxidative stress indices like glutathione, total ascorbic acid, total –SH, superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione –s-transferase levels remainedaltered by 2,4-D feeding. Contrarily increased activities of glycogen and lipid peroxidation (LPO) levels were confirmed by administration of toxicant in a dose dependent manner.These consequences were supported by hepato-histopathological study. However, melatonin administration to 2,4-D treated mice mitigated these metabolic, oxidativestress  as well as histopathological effects and  it is hence concluded that melatonin ameliorates 2,4-D induced toxicity in this vital organdue to its antioxidant properties.

Key Words: Herbicide, Hepatotoxicity, Biochemical and Antioxidant indices, Histopathology, Liver, Mice.

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