ISSN 0976-4550  
DOI: 10.21276/Ijabpt  
 
 
 
Peer Reviewed Online International Journal of Applied Biology and Pharmaceutical Technology www.ijabpt.com
 

ISOLATION AND MOLECULAR IDENTIFICATION OF BACTERIA BY 16SRNA AND POLYMERASE CHAIN REACTION IN DIFFERENT WATER SAMPLES  Page No 52 to 62, Volume-8, Issue-3, July-Sept-2017  

Wadhah Kadhim Hamzah and Mohamed Yahya Khan

Anibiogram data of all the 10 isolates that grew on EMB agar and identified by biochemical tests were also cheked for antibiotic susceptibility and antibiotic resistance using antibiotics.All these isolates were confirmed upto genus level using IMViC and other biochemical test such as urease, catalase, H2S production and sugar fermentation.Gentamycin, streptomycin, oflaxacin, norfloxacin and amoxicillin. Antibiotic susceptibility testing (AST) is usually carried out to determine which antibiotic will be most successful in treating coliform bacteriainfection in vivo. Testing for antibiotic sensitivity is often done by the Kirby-Bauer method. If the bacteria are sensitive to the antibiotic, a clear ring, or zone of inhibition, is seen around the disc indicating poor growth. One isolate WMF1 was identified using molecular (16sr RNA) as E. coli Escherichia fergusonii.Genomic DNA was isolated using kit from xcelris for detection of Lac Z as the β-galactosidase gene and uidA beta-D-glucuronidase ofEscherichia coliand detection of two genes from WMF 1 showed the presence of two genes. Lac Z corresponding to 500 bp and uidA gene corresponding to 147 bp confirming the presence of substrate utilizing genes in E. coli WMF1.

Key words: 16s RNA, PCR, LacZ gene, Antibiotic susceptibility

Pdf
 
 
 
Copyright @ 2010 www.ijabpt.com All rights reserved
Web Counter